(D) Myeloid cell (CD33 +), (E) T cell (CD3 +) and (F) B cell (CD19 +) chimerism in NSG mice bone marrow 4 months after transplantation (n=4 mice per group). (C) The percentage of human CD45 + cells in BM at 4 months after transplantation of NSG mice with the progeny of 50,000 CB CD34 + cells that were treated with vehicle, Eupalinilide E, UM171, alone or in combination for 7 days (n= 4 mice per group). BM cells from mice that did not undergo transplantation were used as the negative control. ![]() (B) Representative FACS plots (from n=4 independent experiments) showing percentage engraftment of vehicle-, Eupalinilide E-, UM171- or Eupalinilide E plus UM171-treated human CB CD34 + cells in the BM of recipient NSG mice. Human engraftment in BM of NSG mice was analyzed 4 months after transplantation. Schematic representation of NSG mice transplanted with progeny of 50,000 vehicle-, Eupalinilide E-, UM171- or Eupalinilide E plus UM171-treated human CB CD34 + cells. While reasons for the disconnect between pHSC and function of HSCs with Eupalinilide E alone cultured CB CD34 + cells is yet to be determined, the data suggest possible future use of Eupalinilide and UM171 together to enhance ex vivo production of CB HSCs for clinical hematopoietic cell transplantation.Įupalinilide E Ex vivo expansion Glycolysis Hematopoietic stem cells UM171.Ĭopyright © 2020. However, Eupalinilide did act in an additive to synergistic fashion with UM171 to enhance ex vivo expansion of both pHSCs, and functionally engrafting HSCs. Lack of effect on engrafting HSCs may be due to a number of possibilities, including down regulation of CXCR4 or of the homing capacity of these treated cells. This is another example of pHSCs not necessarily recapitulating functional activity of these cells. Eupalinilide E ex-vivo enhanced phenotyped (p) HSCs and glycolysis of CD34 + cells isolated 7 days after culture as measured by extracellular acidification rate, but did not alone show enhanced NSG engrafting capability of HSCs as determined by chimerism and numbers of SCID Repopulating cells, a quantitative measure of functional human HSCs. Electronic address: E was assessed for ex-vivo expansion activity on hematopoietic stem cells (HSCs) from human cord blood (CB) CD34 + cells in serum-free, SCF, TPO and FL stimulated 7 day cultures.
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